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Molecular cloning : a laboratory manual
    Green, Michael R., (Michael Richard) 1954-
Pages: 3 Volume :
ISBN: 9781936113422
Item info: 6 copies available.
6 copies total in all locations. 
Holdings
Call number Copies Material Location
572.8 G795m v.1 1 Reserve Book Library 3rd. floor, RES bookshelf
  1 Circulate Book Faculty of Health Education
572.8 G795m v.2 1 Reserve Book Library 3rd. floor, RES bookshelf
  1 Circulate Book Faculty of Health Education
572.8 G795m v.3 1 Reserve Book Library 3rd. floor, RES bookshelf
  1 Circulate Book Faculty of Health Education
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Personal Author: Green, Michael R., (Michael Richard) 1954-
Title: Molecular cloning : a laboratory manual / Michael R. Green, Joseph Sambrook.
Edition: Fourth Edition.
Physical descrip: 3 Volume : illustration (some color) ; 29 cm.
Contents Note: Volume 1: Part 1. Essentials: 1. Isolation and Quantification of DNA - 2. Analysis of DNA - 3. Cloning and Transformation with Plasmid Vectors - 4. Gateway Recombinational Cloning - 5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors - 6. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells - 7. Polymerase Chain Reaction - 8. Bioinformatics
Contents Note: Volume 2: Part 2. Analysis and Manipulation of DNA and RNA: 9. Quantification of DNA and RNA by Real-Time Polymerase Chain Reaction - 10. Nucleic Acid Platform Technologies - 11. DNA Sequencing - 12. Analysis of DNA Methylation in Mammalian Cells - 13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes - 14. Methods for In Vitro Mutagenesis -- Part 3. Introducing Genes into Cells: 15. Introducing Genes into Cultured Mammalian Cells - 16. Introducing Genes into Mammalian Cells: Viral Vectors
Contents Note: Voleme 3: Part 4. Gene Expression: 17. Analysis of Gene Regulation Using Reporter Systems - 18. RNA Interference and Small RNA Analysis - 19. Expressing Cloned Genes for Protein Production, Purification, and Analysis -- Part 5. Interaction Analysis: 20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function - 21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP) - 22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
Summary: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Mol.
Subject term: Molecular cloning--Laboratory manuals
Added author: Sambrook, Joseph,
Electronic access: http://library.uksw.edu/TOC-PDF/2024/24020030.pdf
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